ArtNr |
AAH-ATH-1-4 |
Hersteller |
Raybiotech
|
Menge |
4 Sample Kit |
Quantity options |
2 Sample Kit
4 Sample Kit
8 Sample Kit
|
Kategorie |
|
Typ |
Array |
Specific against |
Human |
Host |
Hamster - Armenian |
Citations |
1. Woroniecki R P, Shatat I F, Supe K, Du Z F, Frederick J K.Urinary Cytokines and Steroid Responsiveness in Idiopathic Nephrotic Syndrome of Childhood. Am J Nephrol. 2008,28:83–90 |
ECLASS 10.1 |
32161000 |
ECLASS 11.0 |
32161000 |
UNSPSC |
41116126 |
Similar products |
IFN-gamma, IL-1 alpha, IL-1 beta, G-CSF, GDNF, RANTES, M-CSF, GM-CSF, MCP-1, ICAM-1, PDGF-BB, Eotaxin-1, TGF beta 1, TGF beta 2, TGF beta 3, TNF beta, MIP-3 alpha, TNF alpha, IL-1 sRI, IL-1 sRII |
Lieferbar |
|
Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Design Principle |
Sandwich-based |
Detection Method |
Chemiluminescence |
Features |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
|
Kit Components |
- Human Atherosclerosis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
|
Number of Targets Detected |
20 |
Other Materials Required |
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
|
Protocol Outline |
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
|
Result Output |
Semi-Quantitative |
Short description |
RayBio C-Series Human Atherosclerosis Antibody Array 1 Kit. Detects 20 Human Atherosclerotic Factors. Suitable for all liquid sample types. |
Solid Support |
Membrane |
Storage |
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months. |
Targets Detected |
CCL11, CCL2, CCL20, CD54, Eotaxin-1, G-CSF, GDNF, GM-CSF, ICAM-1, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-1 R1, IL-1 R2, M-CSF, MCP-1, MIP-3 alpha, PDGF-BB, RANTES, TGF beta 1, TGF beta 2, TGF beta 3, TNF alpha, TNF beta, TNFSF1B |
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