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Human Apoptosis Array G1

Human Apoptosis Array G1

ArtNr	AAH-APO-G1-4
Hersteller	Raybiotech
Menge	4 Sample Kit
Quantity options	4 Sample Kit 8 Sample Kit
Kategorie	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Cholesterol & Lipid Metabolism Neural Mitochondrial Function Molecular Targets for Neuroscience Targets for Rodent Neurosciences Aging & Neurological Disorders Protein Aggregation, Clearance & Misfolding By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Cell Biology Obesity Cell Status Apoptosis Autophagy Senescence Cardiovascular Research Ischemia & Reperfusion Injury Enzymes Fatty Acid Synthesis Arrays By Organ Lung Cancer Bladder Cancer Colorectal Cancer Breast Cancer Testicular & Prostate Cancer Neuronal Cell Markers Antibody Arrays
Typ	Array
Specific against	Human
Host	Hamster - Armenian
Citations	1. The Presence of HIV-1 Tat Second Exon Delays Fas-Mediated Apoptosis in CD4+ T lymphocytes: a Potential Mechanism for Persistent Viral Production 2. Ibrahim M., et al. alpha-Mangostin from Cratoxylum arborescens demonstrates apoptogenesis in MCF-7 with regulation of NF-kappaB and Hsp70 protein modulation in vitro, and tumor reduction in vivo. Drug Des Devel Ther. 2014 Sep 27,8:1629-47. doi: 10.2147/DDDT.S66105
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Similar products	p53, Survivin, IGFBP-5, p21, p27, IGFBP-2, BID, XIAP, CD40, DR6, Fas, HSP70, IGF-1, CD40 Ligand, HSP60, BIM, HTRA, HSP27, IGFBP-1, TNF beta, TNF alpha, IGFBP-3, Fas Ligand, Caspase 8, Caspase 3, IGF-2, TRAIL R1, TRAIL R4, bax, SMAC, IGFBP-4, bcl-w, TRAIL R2, TRAIL R3, sTNFRI, sTNFRII, IGFBP-6, cIAP-2, bcl-2, bad, Cytochrome-C, IGF-1sR, livin
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Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Detection Method	Fluorescence Laser Scanner
Features	High sensitivity and specificity Low sample volume (10-100 ul per array) Large dynamic range of detection Compatible with most sample types Test 4 or 8 samples on each slide Suitable for high-throughput assays
Kit Components	Human Apoptosis Antibody Array G1 Slide(s) Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated Fluor Lysis Buffer Protease Inhibitor Cocktail Adhesive Plastic Strips 30 ml Centrifuge Tube Manual
Number of Targets Detected	43
Other Materials Required	Small plastic boxes or containers Pipettors, pipette tips and other common lab consumables Orbital shaker or oscillating rocker Aluminum foil Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline	Dry the glass slide Block array surface Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with Streptavidin-Conjugated Fluor Disassemble the glass slide Scan with a gene microarray laser scanner Perform densitometry and analysis
Result Output	Semi-Quantitative
Secondary categories	Products///Functional Assays///Apoptosis Assays
Short description	RayBio G-Series Human Apoptosis Antibody Array 1 Kit. Detects 43 Human Apoptotic Factors. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Solid Support	Glass Slide
Storage	For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20C and all other reagents undiluted at 4C for no more than 3 months.
Targets Detected	4-1BB, Apo-1, bad, bax, bcl-2, bcl-w, BID, BIM, BIRC5, Caspase-3, Caspase-8, CD40, CD40 Ligand, cIAP-2, Cytochrome C, DR4, DR5, DR6, Fas, Fas Ligand, HSP27, HSP60, HSP70, HTRA, IGF-1, IGF-1 R, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, Livin, p21, p27, p53, SMAC, Survivin, TNF alpha, TNF beta, TNF RI, TNF RII, TNFRSF10A, TNFRSF10B, TNFRSF10D, TNFRSF21, TNFRSF5, TNFRSF6, TNFRSF9, TNFSF1B, TNFSF5, TNFSF6, TRAIL R1, TRAIL R2, TRAIL R3, TRAIL R4, XIAP

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