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Human Angiogenesis Array C2

Human Angiogenesis Array C2

ArtNr	AAH-ANG-2-4
Hersteller	Raybiotech
Menge	4 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit
Kategorie	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Cholesterol & Lipid Metabolism By Cell Type Blastoma Carcinoma Hematology Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Movement Cell Adhesion Cell Culture Cytokines Adenocarcinoma Arrays By Organ Bladder Cancer Breast Cancer Brain Cancer Antibody Arrays
Typ	Array
Specific against	Human
Host	Hamster - Armenian
Citations	1. Moon MH, Kim SY, Kim YJ, Kim SJ, et al. Human Adipose Tissue-Derived Mesenchymal Stem Cells Improve Postnatal Neovascularization in a Mouse Model of Hindlimb Ischemia. Cell Physiol Biochem. 2006, 17:279-290. 2. Raghu H., Lakka S., Gondi C., Mohanam S., et al. Suppression of uPA and uPAR Attenuates Angiogenin Mediated Angiogenesis in Endothelial and Glioblastoma Cell Lines. PLOS One, Published: August 27, 2010. DOI: 10.1371/journal.pone.0012458
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Similar products	Endostatin, IL-10, IL-1 alpha, IL-1 beta, MMP-1, MMP-9, VEGFR2, IL-4, G-CSF, IL-2, MCP-3, GM-CSF, PECAM-1, MCP-4, I-TAC, I-309, VEGFR3, TNF alpha, Angiopoietin 2, uPAR, Tie-2, Angiostatin, Angiopoietin 1
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Short description	RayBio C-Series Human Angiogenesis Antibody Array 2 Kit. Detects 23 Human Angiogenic Factors. Suitable for all liquid sample types.
Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected	23
Target Names	Angiopoietin-1, Angiopoietin-2, Angiostatin, Endostatin, GCSF, GM-CSF, I-309 (TCA-3/CCL1), IL-10, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-4, I-TAC (CXCL11), MCP-3 (MARC/CCL7), MCP-4 (CCL13), MMP-1, MMP-9, PECAM-1 (CD31), Tie-2, TNF alpha, uPAR, VEGFR2, VEGFR3
Targets Detected	Angiopoietin-1, Angiopoietin-2, Angiostatin, CCL1, CCL13, CCL7, CD31, CXCL11, Endostatin, G-CSF, GM-CSF, I-309, I-TAC, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-2, IL-4, MARC, MCP-3, MCP-4, MMP-1, MMP-9, PECAM-1, TCA-3, Tie-2, TNF alpha, uPAR, VEGFR2, VEGFR3
Suggested Applications	Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components	Human Angiogenesis Antibody Array C2 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated HRP Detection Buffer C Detection Buffer D Lysis Buffer 8-Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with HRP-Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Storage	For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support	Membrane
Design Principle	Sandwich-based
Result Output	Semi-Quantitative
Detection Method	Chemiluminescence
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation	1. Moon MH, Kim SY, Kim YJ, Kim SJ, et al. Human Adipose Tissue-Derived Mesenchymal Stem Cells Improve Postnatal Neovascularization in a Mouse Model of Hindlimb Ischemia. Cell Physiol Biochem. 2006, 17:279-290. 2. Raghu H., Lakka S., Gondi C., Mohanam S., et al. Suppression of uPA and uPAR Attenuates Angiogenin Mediated Angiogenesis in Endothelial and Glioblastoma Cell Lines. PLOS One, Published: August 27, 2010. DOI: 10.1371/journal.pone.0012458

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