ArtNr |
AAH-ANG-1000-8 |
Hersteller |
Raybiotech
|
Menge |
8 Sample Kit |
Quantity options |
2 Sample Kit
4 Sample Kit
8 Sample Kit
|
Kategorie |
|
Typ |
Array |
Specific against |
Human |
Host |
Hamster - Armenian |
Citations |
1. Moon MH, Kim SY, Kim YJ, Kim SJ, et al. Human Adipose Tissue-Derived Mesenchymal Stem Cells Improve Postnatal Neovascularization in a Mouse Model of Hindlimb Ischemia. Cell Physiol Biochem. 2006, 17:279-290. 2. Konig J., et al. Placental mesenchymal stromal cells derived from blood vessels or avascular tissues: What is the better choice to support endothelial cell function?. Stem Cells and Development, Sept 2014 epub ahead of print. doi:10.1089/scd.2014.0115) |
ECLASS 10.1 |
32161000 |
ECLASS 11.0 |
32161000 |
UNSPSC |
41116126 |
Lieferbar |
|
Number of Targets Detected |
43 |
Species Detected |
Human |
Compatible Sample Types |
Cell Culture Supernatants Cell Lysates Plasma Serum Tissue Lysates |
Design Principle |
Sandwich-based |
Method of Detection |
Chemiluminescence |
Quantitative/Semi-Quantitative |
Semi-Quantitative |
Solid Support |
Membrane |
Gene Symbols |
ANG | ANGPT1 | ANGPT2 | CCL1 | CCL13 | CCL2 | CCL5 | CCL7 | COL18A1 | CSF2| CSF3 | CXCL1 | CXCL11 | CXCL2 | CXCL3 | CXCL5 | CXCL8 | EGF | FGF2 | FIGF | FLT4 | IFNG | IGF1 | IL10 | IL1A | IL1B | IL2 | IL4 | IL6 | KDR | LEP | MMP1 | MMP9 | PDGFB | PECAM1 | PGF | PLAUR | PLG | TEK | TGFB1 | THPO | TIMP1 | TIMP2 | TNF | TPO | VEGFA |
Application Information |
Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker/ Key Factor Screening Identifying Key Factors Confirming a Biological Process |
Kit Components |
Human Angiogenesis Antibody Array C1000 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin- Conjugated H R P Detection Buffer C Detection Buffer D Lysis Buffer8- Well Incubation Tray Plastic Sheets Array Templates Manual |
Other Materials Required |
Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as U V P's Chemi Doc- It® or Epi Chem I I Benchtop Darkroom or G E's Image Quant™ L A S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system. |
Protocol Outline |
Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with H R P- Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis |
Storage/Stability |
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.