Vergleich

Human AKI Array C1

ArtNr AAH-AKI-1-4
Hersteller Raybiotech
Menge 4 Sample Kit
Quantity options 2 Sample Kit 4 Sample Kit 8 Sample Kit
Kategorie
Typ Array
Specific against Human (Homo sapiens)
Host Hamster - Armenian
Citations Chen T., et al., Elevated Urinary Levels of Cystatin C and Neutrophil Gelatinase-Associated Lipocalin in Henoch-Scho¨ nlein Purpura Patients with Renal Involvement. PLOS One. Published: June 25, 2014 DOI: 10.1371/journal.pone.0101026
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Similar products TIMP-1, VCAM-1, HGF, MIF, VEGF-A, B2M, NGAL, MCP-1, CXCL16, Cystatin C, KIM-1, Liver-type fatty acid binding protein (L-FABP), Albumin (ALB), Osteopontin (Osteopontin), Trefoil Factor 3 (TFF3), Clusterin (CLU), Osteoactivin, sTNFRI, Calbindin (CALB1), Interferon gamma-induced protein 10 (IP-10)
Versandbedingung Gekühlt
Lieferbar
Number of Targets Detected
20
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle
Sandwich-based
Solid Support
Membrane
Kit Components
  • Human Acute Kidney Injury (AKI) Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Detection Method
Chemiluminescence
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Result Output
Semi-Quantitative
Short description
RayBio C-Series Human Acute Kidney Injury Antibody Array 1 Kit. Detects 20 Human Cytokines. Suitable for all liquid sample types.
Storage
For best results, store the entire kit frozen a -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer a -20C and all other reagents undiluted at 4C for no more than 3 months.
Targets Detected
Albumin, Beta-2 Microglobulin, Calbindin, CCL2, CD106, Clusterin, CXCL10, CXCL16, Cystatin C, FABP1, GPNMB, HGF, IP-10, KIM-1, Lipocalin-2, MCP-1, MIF, NGAL, Osteoactivin, Osteopontin, SPP1, TFF3, TIM-1, TIMP-1, TNF RI, Trefoil Factor 3, VCAM-1, VEGF-A

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 4 Sample Kit
Lieferbar: In stock
lieferbar

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