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Human Obesity Array C1

Human Obesity Array C1

ArtNr	AAH-ADI-1-2
Hersteller	Raybiotech
Menge	2 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit
Kategorie	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Regulation of Appetite Neural Signaling Neural Adhesion Molecules By Cell Type Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Adenocarcinoma Mesothelioma Endocrine Regulation of Lipid Metabolism Senescence Enzymes Other Fatty Acid Enzymes Arrays By Organ Lung Cancer Bladder Cancer Colorectal Cancer Breast Cancer Antibody Arrays
Typ	Array
Specific against	Human
Host	Hamster - Armenian
Citations	Bauer D., Mazzio E., Soliman K., Taka E., Oriaku E., et al. Diallyl Disulfide Inhibits TNFalpha-induced CCL2 Release by MDA-MB-231 Cells. ANTICANCER RESEARCH 34: 2763-2770 (2014)
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Similar products	CRP, Adiponectin, IFN-gamma, IL-10, IL-1 alpha, IL-1 beta, IL-6, Insulin, Leptin, Osteoprotegerin, TIMP-1, IL-12, IL-8, IL-6, SDF-1, Resistin, MIF, LIF, ANGPTL4, IGFBP-2, VEGF-A, PARC, XEDAR, Fas, IGF-1, RANTES, OSM, Growth Hormone, M-CSF, MCP-3, IL-6 sR, IL-11, MCP-1, ENA-78, Lymphotactin, TECK, TIMP-2, IP-10, PDGF-AA, PDGF-BB, MIP-1 beta, 4-1BB, PDGF-AB, HCC-4, Adipsin, IGFBP-1, TGF beta 1, FGF-6, TNF alpha, IGFBP-3, Angiopoietin 2, Leptin R, AgRP, Serum Amyloid A, PAI-I, sTNFRI, sTNFRII, IL-1 R4, IL-1 sRI, Angiopoietin 1, IGF-1 sR, ACE-2, MSP-alpha
Lieferbar
Number of Targets Detected	62
Species Detected	Human
Compatible Sample Types	Cell Culture Supernatants Cell Lysates Plasma Serum Tissue Lysates
Design Principle	Sandwich-based
Method of Detection	Chemiluminescence
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Membrane
Gene Symbols	ACE2 \| ADIPOQ \| AGRP \| ANGPT1 \| ANGPT2 \| ANGPTL4 \| CCL16 \| CCL18 \| CCL2 \| CCL25\| CCL4 \| CCL5 \| CCL7 \| CFD \| CRP \| CSF1 \| CXCL10 \| CXCL12 \| CXCL5 \| CXCL8 \| EDA2R \| FAS \| FGF6 \| GH1 \| IFNG \| IGF1 \| IGF1R \| IGFBP1 \| IGFBP2 \| IGFBP3 \| IL10 \| IL11 \| IL12A \| IL1A \| IL1B \| IL1R1 \| IL1RL1 \| IL6 \| IL6R \| INS \| LEP \| LEPR \| LIF \| MIF \| MST1 \| OSM \| PDGFA \| PDGFB \| RETN \| SAA1 \| SERPINE1 \| TGFB1 \| TIMP1 \| TIMP2 \| TNF \| TNFRSF11B \| TNFRSF1A \| TNFRSF1B \| TNFRSF9 \| TNFSF9 \| VEGFA \| XCL1
Application Information	Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker/ Key Factor Screening Identifying Key Factors Confirming a Biological Process
Kit Components	Human Obesity Antibody Array C1 Membranes Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin- Conjugated H R P Detection Buffer C Detection Buffer D Lysis Buffer 8- Well Incubation Tray Plastic Sheets Array Templates Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as U V P's Chemi Doc- It® or Epi Chem I I Benchtop Darkroom or G E's Image Quant™ L A S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with H R P- Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Product Features	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
Storage/Stability	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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