ArtNr |
QAE-CYT-1-1 |
Hersteller |
Raybiotech
|
Menge |
8 Sample Kit |
Kategorie |
|
Typ |
Assay |
Specific against |
Equine |
ECLASS 10.1 |
32161000 |
ECLASS 11.0 |
32161000 |
UNSPSC |
41116126 |
Similar products |
IL-10, IL-1 alpha, VEGF, IL-4, IL-8, IL-2, IFN gamma, IL-15, MCP-1, IL-1R alpha |
Lieferbar |
|
Short description |
Quantibody Equine (horse) Cytokine Array 1 Kit. Detects 10 Equine Cytokines. Suitable for all liquid sample types. |
Features |
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 uL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
|
Number of Targets Detected |
10 |
Target Names |
IFN-gamma, IL-1 alpha (IL-1 F1), IL-1 Ra (IL-1 F3), IL-2, IL-4, IL-8 (CXCL8), IL-10, IL-15, MCP-1 (CCL2), VEGF-A |
Targets Detected |
CCL2, CXCL8, IFN-gamma, IL-1 alpha, IL-1 F1, IL-1 F3, IL-1 Ra, IL-10, IL-15, IL-2, IL-4, IL-8, MCP-1, VEGF-A |
Suggested Applications |
Multiplexed Protein Detection, Biomarker Screening, Identifying Key Factors, Confirming Biological Process, Biomarker Validation, Validation of Antibody Array Results, Quantitative Protein Detection, Establishing Normal Range |
Kit Components |
- Quantibody Bovine Cytokine Array 1 Slide
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- 30 ml Centrifuge Tube
- Adhesive Plastic Strips
- Manual
|
Other Materials Required |
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
|
Protocol Outline |
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
|
Storage |
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20C and all other reagents undiluted at 4C for no more than 3 months. |
Solid Support |
Glass Slide |
Design Principle |
Sandwich-based |
Result Output |
Quantitative |
Detection Method |
Fluorescence Laser Scanner |
Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.