Application notes: |
ELISA (recommended work dilution= 1:10e6(at least detecting 10ng/ml)), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Enzyme Immunoassay (EIA) (recommended work dilution= Sensitivity (100pg/ml)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3-4.Positive control, Hela and MD231 whole cell lysate, Lane 5.Negative control. The membrane blot was probed with antiVisfatin primary antibody(1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular Visfatin from human and mouse cells (52 kDa). |
Product Description |
Visfatin also designated Pre-B cell-enhancing factor (PBEF) or Nicotinamide hosphoribosyltransferase (Nampt) , belongs to the NAPRTase family of proteins. Visfatin is an inflammatory cytokine that plays a requisite role in the delayed neutrophil apoptosis of sepsis. Visfatin exerted insulin-mimetic effects in cultured cells and lowered plasma glucose levels in mice. It was found that visfatin binds to and activates the insulin receptor, albeit in a different place than insulin. Furthermore, Visfatin may be involved in enhancing the effect of IL-7 and SCF on the formation of early B-lineage precursor colonies. More recently, several groups have reported the crystal structure of Nampt/PBEF/visfatin and they all show that this protein is a dimeric type II phosphoribosyltransferase enzyme involved in NAD biosynthesis pathway. Visfatin is a cytoplasmic protein with a molecular weight of 52 kDa composed of 473-amino acid. It has a hydrophobic N terminus, 2 N-glycosylation sites, several putative phosphorylation sites, and 6 cysteines. Visfatin is expressed primarily in bone marrow, muscle and liver tissue but can also be detected in placenta, lung, kidney and heart tissue. |