Application notes: |
ELISA (recommended work dilution= 1: 1, 000-2, 000), Western Blotting (recommended work dilution= 2-5 ug/ml), Immunoprecipitation |
Product Description |
Microtubule-associated protein, tau is abnormally hyperphosphorylated in the brain of patients with Alzheimer's disease, and is the major protein subunit of paired helical filaments. There is also a significant pool of non-paired helical filament abnormally phosphorylated tau in Alzheimer's disease brain. Tau is a family of six isoforms, derived from a single gene by mRNA splicing. Tau protein is produced by a single gene expressed predominantly in neurons. They vary in size from 352-441 amino acides. In Alzheimer disease Tau is hyperphosphorylated, containing 3-4 fold more phosphoate/mole of the protein than the normal tau. Recent investigations show that MARK and PKA phosphorylate several sites within the repeats (notably the KXGS motifs including Ser262, Ser324, and Ser356, plus Ser320), in addition PKA phosphorylates some sites in the flanking domains, notably Ser214. This type of phosphorylation strongly reduces tau's affinity for microtubules, and at the same time inhibits tau's assembly into PHFs (Paired helical filament). |
Immunogen |
Immunogen was synthetic peptide derived from human Tau. This antibody was obtained from a mice immunized with the immunogen, then fusion, selection and identification. The identified clone was called as 17D3.G7. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells deriveded from the immunized mouse. The IgG fraction of cell culture supernatant was purified by Protein G affinity chromatography. |