Rabbit Anti-Human EGF [+Biotin]

ELISA (dilution: 0.1, 0.2ug/ml), Western Blotting

EGF

Reconstitute with 1.0ml sterile PBS containing 0.1% Bovine Serum Albumin.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.

This product is specific for mouse EGF (Epidermal Growth Factor), a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, which is expressed by a variety of cells.The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the 170kDa EGF receptor (EGFR), which also binds other EGF-like ligands with high affinity, including TGF-α, VGF (vaccinia virus growth factor) and Betacellulin, produced by certain tumour cells. Receptor activation through EGF binding triggers several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways.

Antisera to human EGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Purified IgG conjugated to Biotin - lyophilized