ArtNr |
UC3-H5141-1mg |
Hersteller |
ACROBiosystems
|
Menge |
1 mg |
Kategorie |
|
Typ |
Proteins Recombinant |
Format |
Powder |
Specific against |
Human |
Host |
E.coli |
Konjugat/Tag |
Unconjugated, HIS |
Purity |
95% |
Citations |
(1) Nam M.J., et al., 2003, Proteomics 3:2108-2115. (2) Setsuie R., et al., 2009, Neurochem. Int. 54:314-321. (3) Iphofer A., et al., 2012, ChemBioChem 13:1416-1420. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
UCHL3,Ubiquitin thioesterase L3 |
Lieferbar |
|
Manufacturers Category |
Protein / Enzymes |
Description |
Human UCH-L3, His Tag (UC3-H5141) is expressed from E. coli cells. It contains AA Glu 2 - Ala 230 (Accession # P15374-1). |
Molecule |
UCH-L3 |
Exp Region |
Glu 2 - Ala 230 |
Storage |
-20℃ |
Shipping |
RT |
Stability |
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Molecular Weight |
27.0 kDa |
Characteristics |
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 27.0 kDa. The protein migrates as 28-30 kDa under reducing (R) condition (SDS-PAGE). |
Endotoxin |
1.0 EU per μg |
Buffer |
50 mM Tris, 150 mM NaCl, pH7.5 |
Background |
Ubiquitin carboxyl-terminal hydrolase isozyme L3 (UCH-L3), a member of peptidase C12 family, is also known as ubiquitin thioesterase L3. Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. UCH-L3 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCH-L3 indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is also required for stress-response retinal, skeletal muscle and germ cell maintenance. Furthermore, UCH-L3 may be involved in working memory and can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders. |
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