PD1 Recombinant Protein

This recombinant protein can be used for WB. For research use only.

Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

42.6 kDa

Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.

Ishida Y., et al., 1992, EMBO J. 11 (11): 3887–95.

Agata Y., et al., 1996, Int. Immunol. 8 (5): 765–72.

NP_005009.2

PDCD1

Homo sapiens

C-Fc Tag

(1） Measured by its binding ability in a functional ELISA. Immobilized Human PD-L1, His Tag at 5 ug/mL (100 ul/well) can bind Human PD-1, Fc Tag (HPLC-verified) with a linear range of 0.05-1 ug/mL.（2） Measured by its binding ability in a functional ELISA. Immobilized Human PD-L2, His Tag at 5 ug/mL (100 ul/well) can bind Human PD-1, Fc Tag with a linear range of 0.02-0.5 ug/mL.