ArtNr |
PRS-40-103-0.002mg |
Hersteller |
ProSci
|
Menge |
0.002 mg |
Kategorie |
|
Typ |
Proteins Recombinant |
Format |
Lyophilized |
Specific against |
other |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
TL2, APO2L, CD253, TRAIL, Apo-2L, Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand |
Lieferbar |
|
Storage Conditions |
The lyophilized TGF beta 1 recombinant protein is stable for at least 2 years from date of receipt at -20˚ C. Reconstituted TGF beta 1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚ C. As with any protein, exposing TGF beta 1 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
Disclaimer |
This product is for research use only. |
Purity |
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μ g (1EU/μ g). |
Background |
The three mammalian isoforms of TGF-b, TGF-b1, b2, b3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-b isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-b1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors. Recombinant human TGF-b1 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond. |
Accession # |
NP_001177871.1 |
Ncbi Gene Id # |
8743 |
Ncbi Official Symbol |
TNFSF10 |
Ncbi Official Full Name |
tumor necrosis factor (ligand) superfamily, member 10 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P50591 |
Sequence |
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS |
Biological Activity |
The ED50 was determined by TGF beta 1’s ability to inhibit the mouse IL - 4 - dependent proliferation of mouse HT - 2 cells is ≤ 0.05 ng/mL, corresponding to a specific activity of ≥ 2 x 107 units/mg |
Protein Gi# |
300193032 |
Peptide Sequence |
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.