ArtNr |
NKM-ATGP4029-250ug |
Hersteller |
NKMAX
|
Menge |
250ug |
Kategorie |
|
Typ |
Proteins |
Applikationen |
SDS-PAGE, Bioactivity |
Specific against |
other |
Konjugat/Tag |
His-Tag |
Purity |
> 90% by SDS - PAGE |
NCBI |
NP_034688 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
iinterleukin 5, Il, Il-5, B-cell growth factor II, BCGF-II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor, TRF, B-cell differentiation factor I, EDF, IL5, TRFB cell differentiation factor I |
Lieferbar |
|
Antigen Species |
Mouse |
Bioactivity Comment Only |
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 1 ng/ml. |
Bioactivity Comment and Pics |
Mouse IL-5 in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 1 ng/ml. |
Bioassay Procedure |
MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG |
Concentration |
0.25mg/ml (determined by Absorbance at 280nm) |
Description |
IL5, also known as interleukin-5, is a member of the hematopoietin receptor superfamily and is comprised of a cytokine-specific alpha chain and the common beta chain that is shared among these cytokines for signaling. This interleukin produced by type-2 T helper cells and mast cells. It related hematopoietic cytokines that are important for allergic inflammation.. It is a lineage-specific cytokine for eosinophilpoiesis and plays an important part in diseases associated with increased eosinophils, such as asthma. In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells. IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release. Recombinant mouse IL-5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
Domain |
21-133aa |
Endotoxin |
< 1 EU per 1ug of protein (determined by LAL method) |
Expression System |
Baculovirus |
Formulation |
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol |
Gene Category |
Immunology |
General References |
Shen HH., et al, (2003) J. Immunol. 170 (6): 3296–3305.; ; Sanderson CJ., et al, (1992) Blood. 79 (12): 3101–3109. |
Molecular Weight |
13.9kDa (119aa) |
Predicted nterm |
Met 21 |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
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