Recombinant human IL-10 protein

Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 range <=5ng/ml.

SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN

IL-10, also known as Interleukin-10, is an anti-inflammatory cytokine. It inhibits the synthesis of pro-inflammatory cytokines like IFN-gamma, IL2, IL3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. This protein has effects in immunoregulation and inflammation and down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. This protein controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling. Recombinant human IL-10, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

< 1 EU per 1ug of protein (determined by LAL method)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Jog NR., et al. (2018) Front Immunol. 9:2198.; ; Harling K., et al. (2019) Cell Mol Immunol. 16:275-287.

Ser19