Recombinant human Transgelin/TAGLN protein

Human

TAGLN is a transformation and shape-change sensitive actin cross-linking/gelling protein that belongs to the calponin family. This protein is expressed abundantly in fibroblasts and smooth muscle. It is involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. During embryogenesis, TAGLN is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. It is downregulated in several transformed cell lines, indicating that a reduction of TAGLN expression may be an early indicator of the onset of transformation. Recombinant human TAGLN protein, fused to his-tag at N-terminus was expressed in E. coli and purified by using conventional chromatography techniques

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 1mM DTT

Kobayashi R., et al. (1994) Biochem Biophys Res Commun. 198(3):1275-80. ; ; Thweatt R., et al. (1992) Biochem Biophys Res Commun. 187(1):1-7. ; ;

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.