Recombinant human Glutaredoxin 3/GLRX3 protein

Human

Glutaredoxin (GRX), also known as thioltransferase, is member of the thiol-disulfide oxidoreductase family. Glutraredoxin catalyzes the reversible reduction of protein-glutathionyl mixed disulfides to free sulfhydryl groups though a monothiol mechanism. Glutaredoxin-3 (Grx3/PICOT) is an essential protein involved in the regulation of signal transduction, for instance during immune cell activation and development of cardiac hypertrophy, presumably in response to redox signals. And it has been shown to interact with PRKCQ. Recombinant human Glutaredoxin-3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Witte S., et al. (2000) J Biol Chem 275(3): 1902-9.; ; Haunhorst P., et al. (2010) Biochem Biophys Res Commun. 394(2):372-6; ;

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.