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Recombinant human Enolase 1/Non-neural enolase protein

ArtNr NKM-ATGP0404-500ug
Hersteller NKMAX
Menge 500ug
Kategorie
Typ Proteins Recombinant
Applikationen SDS-PAGE
Specific against other
Konjugat/Tag Non-Tagged
Purity > 90% by SDS-PAGE
NCBI NP_001419
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Enolase1, Non-neural enolase, Non-Neuronal Enolase (NNE), Enolase-1, ENO1, MBP-1, MYC promoter-binding protein 1, PPH, tau-crystallin, 2 phospho D glycerate hydro lyase, Alpha enolase, C myc promoter binding protein, EC 4.2.1.11, ENO1L1, Enolase 1, Enolase 1 (alpha), Enolase 1 (alpha) like 1, MBP 1, MBP1, MBPB1, MPB 1, MPB1, PPH, MYC promoter binding protein 1, NNE, Non neural enolase, Phosphopyruvate hydratase, Plasminogen binding protein,
Lieferbar
Antigen Species
Human
Bioactivity Comment Only
Specific activity is > 20, 000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.
Concentration
1mg/ml (determined by Bradford assay)
Description
Alpha-enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of alpha-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma. Recombinant human alpha-enolase was expressed in E. coli and purified by using conventional chromatography.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Expression System
E.coli
Formulation
Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 1mM MgSO4, 10% glycerol
Gene Category
Metabolism
General References
Das R., et al. (2009) Blood. 113(22):5371-2.; ; ueno NT., et al. (2008) Cancer Res. 68(22):9302-10.; ;
Molecular Weight
47.1 kDa (434aa) confirmed by MALDI-TOF
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 500ug
Lieferbar: In stock
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