ArtNr |
NKM-ATGP0297-20ug |
Hersteller |
NKMAX
|
Menge |
20ug |
Kategorie |
|
Typ |
Proteins Recombinant |
Applikationen |
SDS-PAGE |
Specific against |
other |
Konjugat/Tag |
His-Tag |
Purity |
> 90% by SDS-PAGE |
Citations |
Jung SH, et al. Integrative proteomic profiling of protein activity and interactions using protein arrays. Mol Cell Proteomics. 2012 Nov;11(11):1167-76.{https://pubmed.ncbi.nlm.nih.gov/22843993/} |
NCBI |
NP_000624 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
B-cell lymphoma protein 2 alpha, B-cell lymphoma protein 2 alpha, B-cell lymphoma protein 2 alpha Apoptosis regulator Bcl 2, Apoptosis regulator Bcl2, AW986256, B cell CLL/lymphoma 2, B cell leukemia/lymphoma 2, Bcl-2, Bcl2, C430015F12Rik, D630044D05Rik, D830018M01Rik, Leukemia/lymphoma, B-cell, 2, Oncogene B-cell leukemia 2. |
Lieferbar |
|
Antigen Species |
Human |
Bioassay Procedure |
MGSSHHHHHH SSGLVPRGSH MAHAGRTGYD NREIVMKYIH YKLSQRGYEW DAGDVGAAPP GAAPAPGIFS SQPGHTPHPA ASRDPVARTS PLQTPAAPGA AAGPALSPVP PVVHLTLRQA GDDFSRRYRR DFAEMSSQLH LTPFTARGRF ATVVEELFRD GVNWGRIVAF FEFGGVMCVE SVNREMSPLV DNIALWMTEY LNRHLHTWIQ DNGGWDAFVE LYGPSMRPLF D |
Concentration |
0.25mg/ml (determined by Bradford assay) |
Description |
Bcl-2, also known as B-cell lymphoma protein 2 alpha, is an anti-apoptotic protein located primarily in the outer mitochondrial membrane that blocks the apoptotic death of some cells such as lymphocytes. BCL-2 is thought to regulate cell death by controlling the mitochondrial membrane permeability during apotosis. Bcl-2 exerts its anti-apoptotic function through inhibiting caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). The Bcl-2 gene has been related with a number of cancers, including melanoma, breast, prostate, and lung carcinomas, as well as schizophrenia and autoimmunity. Recombinant Bcl-2 protein was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. |
Domain |
1-211aa |
Expression System |
E.coli |
Formulation |
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 2mM DTT |
Gene Category |
Cancer |
General References |
Zamzami N., et al. (1998) Oncogene. 16(17):2265-82.; ; Fernandez-Sarabia MJ., et al. (1993) Nature. 366(6452):274-5.; ; |
Molecular Weight |
25.4 kDa (231aa) confirmed by MALDI-TOF |
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