Biotinylated Human Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer Protein, His,Avitag™&Tag Free

Biotin-labeled, C-10xHis & C-Avi | Native

-20°C

Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His, Avitag&Tag Free (IT1-H82W1) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1) (Accession # P13612-1 (ITGA4) & P05556-1 (ITGB1)).

Integrin alpha 4 beta 1

Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His, Avitag&Tag Free, produced by co-expression of ITGA4 and ITGB1, has a calculated MW of 113.2 kDa (ITGA4) and 83.7 kDa (ITGB1). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The ITGA4 subunit is composed of a heavy chain (Tyr 34 - Arg 591, calculated MW 61.1 kDa) and a light chain (Ser 592 - Thr 977, calculated MW 52.1 kDa). Consequently ITGA4 migrates as 65-80 kDa, and ITGB1 as 90-150 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

50 mM Tris, 150 mM NaCl, pH7.5

trehalose