ArtNr |
CSB-YP009196HU-20 |
Hersteller |
Cusabio
|
Menge |
20ug |
Quantity options |
1mg
10ug
100ug
20ug
200ug
50ug
500ug
|
Kategorie |
|
Typ |
Proteins Recombinant |
Format |
Liquid or Lyophilized powder |
Specific against |
other |
Host |
Yeast |
Purity |
Greater than 90% as determined by SDS-PAGE. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Lieferbar |
|
Research Areas |
Cardiovascular |
Uniprot ID |
P54803 |
Gene Names |
GALC |
Organism |
Homo sapiens (Human) |
AA Sequence |
YVLDDSDGLGREFDGIGAVSGGGATSRLLVNYPEP YRSQILDYLFKPNFGASLHILKVEIGGDGQTTDGT EPSHMHYALDENYFRGYEWWLMKEAKKRNPNITLI GLPWSFPGWLGKGFDWPYVNLQLTAYYVVTWIVGA KRYHDLDIDYIGIWNERSYNANYIKILRKMLNYQG LQRVKIIASDNLWESISASMLLDAELFKVVDVIGA HYPGTHSAKDAKLTGKKLWSSEDFSTLNSDMGAGC WGRILNQNYINGYMTSTIAWNLVASYYEQLPYGRC GLMTAQEPWSGHYVVESPVWVSAHTTQFTQPGWYY LKTVGHLEKGGSYVALTDGLGNLTIIIETMSHKHS KCIRPFLPYFNVSQQFATFVLKGSFSEIPELQVWY TKLGKTSERFLFKQLDSLWLLDSDGSFTLSLHEDE LFTLTTLTTGRKGSYPLPPKSQPFPSTYKDDFNVD YPFFSEAPNFADQTGVFEYFTNIEDPGEHHFTLRQ VLNQRPITWAADASNTISIIGDYNWTNLTIKCDVY IETPDTGGVFIAGRVNKGGILIRSARGIFFWIFAN GSYRVTGDLAGWIIYALGRVEVTAKKWYTLTLTIK GHFASGMLNDKSLWTDIPVNFPKNGWAAIGTHSFE FAQFDNFLVEATR |
Expression Region |
43-685aa |
Sequence Info |
Full Length of Mature Protein |
Source |
Yeast |
Tag Info |
N-terminal 6xHis-tagged |
MW |
74.8 kDa |
Alternative Name(s) |
Galactocerebroside beta-galactosidase Galactosylceramidase Galactosylceramide beta-galactosidase |
Relevance |
Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon. |
Reference |
Structure and organization of the human galactocerebrosidase (GALC) gene.Luzi P., Rafi M.A., Wenger D.A.Genomics 26:407-409(1995) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Form |
Liquid or Lyophilized powder |
Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon. |
Involvement in disease |
Leukodystrophy, globoid cell (GLD) |
Subcellular Location |
Lysosome |
Protein Families |
Glycosyl hydrolase 59 family |
Tissue Specificity |
Detected in urine. Detected in testis, brain and placenta (at protein level). Detected in kidney and liver. |
Tag Information |
N-terminal 6xHis-tagged |
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