ArtNr |
CSB-EP889918MO-1 |
Hersteller |
Cusabio
|
Menge |
1mg |
Quantity options |
1mg
100ug
20ug
|
Kategorie |
|
Typ |
Proteins Recombinant |
Format |
Liquid or Lyophilized powder |
Specific against |
other |
Purity |
Greater than 85% as determined by SDS-PAGE. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Lieferbar |
|
Research Areas |
Metabolism |
Uniprot ID |
Q9R078 |
Gene Names |
Prkab1 |
Organism |
Mus musculus (Mouse) |
AA Sequence |
GNTSSERAALERQAGHKTPRRDSSGGAKDGDRPKI LMDSPEDADIFHSEEIKAPEKEEFLAWQHDLEAND KAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLT RSQNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPI VTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSDV SELSSSPPGPYHQEPYMSKPEERFKAPPILPPHLL QVILNKDTGISCDPALLPEPNHVMLNHLYALSIKD GVMVLSATHRYKKKYVTTLLYKPI |
Expression Region |
2-270aa |
Sequence Info |
Full Length of Mature Protein |
Source |
E.coli |
Tag Info |
N-terminal 6xHis-tagged |
MW |
36.1 kDa |
Alternative Name(s) |
AMPK subunit beta-1 (AMPKb) |
Relevance |
Non-catalytic subunit of AMP-activated protein kinase, an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha and gamma subunits. |
Reference |
Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry. Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) |
Purity |
Greater than 85% as determined by SDS-PAGE. |
Form |
Liquid or Lyophilized powder |
Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Tag Information |
N-terminal 6xHis-tagged |
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