Recombinant Mouse Receptor-interacting serine/threonine-protein kinase 1(Ripk1)

Cancer

Not Test

Mus musculus (Mouse)

MQPDMSLDNIKMASSDLLEKTDLDSGGFGKVSLCY HRSHGFVILKKVYTGPNRAEYNEVLLEEGKMMHRL RHSRVVKLLGIIIEEGNYSLVMEYMEKGNLMHVLK TQIDVPLSLKGRIIVEAIEGMCYLHDKGVIHKDLK PENILVDRDFHIKIADLGVASFKTWSKLTKEKDNK QKEVSSTTKKNNGGTLYYMAPEHLNDINAKPTEKS DVYSFGIVLWAIFAKKEPYENVICTEQFVICIKSG NRPNVEEILEYCPREIISLMERCWQAIPEDRPTFL GIEEEFRPFYLSHFEEYVEEDVASLKKEYPDQSPV LQRMFSLQHDCVPLPPSRSNSEQPGSLHSSQGLQM GPVEESWFSSSPEYPQDENDRSVQAKLQEEASYHA FGIFAEKQTKPQPRQNEAYNREEERKRRVSHDPFA QQRARENIKSAGARGHSDPSTTSRGIAVQQLSWPA TQTVWNNGLYNQHGFGTTGTGVWYPPNLSQMYSTY KTPVPETNIPGSTPTMPYFSGPVADDLIKYTIFNS SGIQIGNHNYMDVGLNSQPPNNTCKEESTSRHQAI FDNTTSLTDEHLNPIRENLGRQWKNCARKLGFTES QIDEIDHDYERDGLKEKVYQMLQKWLMREGTKGAT VGKLAQALHQCCRIDLLNHLIRASQS

1-656aa

87.8 kDa

Serine-threonine kinase which transduces inflammatory and cell-death signals following death receptors ligation, activation of pathogen recognition receptors, and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.

Greater than 85% as determined by SDS-PAGE.

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.

N-terminal 6xHis-SUMO-tagged