ArtNr |
CSB-EP351974SKX-20 |
Hersteller |
Cusabio
|
Menge |
20ug |
Kategorie |
|
Typ |
Proteins Recombinant |
Format |
Liquid or Lyophilized powder |
Specific against |
other |
Host |
E.coli |
Purity |
Greater than 90% as determined by SDS-PAGE. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Lieferbar |
|
Research Areas |
Others |
Uniprot ID |
P64078 |
Gene Names |
eno |
Organism |
Staphylococcus aureus (strain Mu50 / ATCC 700699) |
AA Sequence |
MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRA LVPSGASTGEHEAVELRDGDKSRYLGKGVTKAVEN VNEIIAPEIIEGEFSVLDQVSIDKMMIALDGTPNK GKLGANAILGVSIAVARAAADLLGQPLYKYLGGFN GKQLPVPMMNIVNGGSHSDAPIAFQEFMILPVGAT TFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGF APKFEGTEDAVETIIQAIEAAGYKPGEEVFLGFDC ASSEFYENGVYDYSKFEGEHGAKRTAAEQVDYLEQ LVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQL VGDDLFVTNTEILAKGIENGIGNSILIKVNQIGTL TETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIA VATNAGQIKTGSLSRTDRIAKYNQLLRIEDELFET AKYDGIKSFYNLDK |
Expression Region |
1-434aa |
Sequence Info |
Full Length |
Source |
E.coli |
Tag Info |
N-terminal 6xHis-SUMO-tagged |
MW |
63.1 kDa |
Alternative Name(s) |
2-phospho-D-glycerate hydro-lyase 2-phosphoglycerate dehydratase |
Relevance |
Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis. |
Reference |
Whole genome sequencing of meticillin-resistant Staphylococcus aureus.Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. Hiramatsu K.Lancet 357:1225-1240(2001) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Form |
Liquid or Lyophilized powder |
Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis. |
Subcellular Location |
Cytoplasm, Secreted, Cell surface |
Protein Families |
Enolase family |
Tag Information |
N-terminal 6xHis-SUMO-tagged |
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