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Recombinant Staphylococcus aureus Enolase(eno)

ArtNr CSB-EP351974SKX-20
Hersteller Cusabio
Menge 20ug
Kategorie
Typ Proteins Recombinant
Format Liquid or Lyophilized powder
Specific against other
Host E.coli
Purity Greater than 90% as determined by SDS-PAGE.
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Lieferbar
Research Areas
Others
Uniprot ID
P64078
Gene Names
eno
Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
AA Sequence
MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRA LVPSGASTGEHEAVELRDGDKSRYLGKGVTKAVEN VNEIIAPEIIEGEFSVLDQVSIDKMMIALDGTPNK GKLGANAILGVSIAVARAAADLLGQPLYKYLGGFN GKQLPVPMMNIVNGGSHSDAPIAFQEFMILPVGAT TFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGF APKFEGTEDAVETIIQAIEAAGYKPGEEVFLGFDC ASSEFYENGVYDYSKFEGEHGAKRTAAEQVDYLEQ LVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQL VGDDLFVTNTEILAKGIENGIGNSILIKVNQIGTL TETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIA VATNAGQIKTGSLSRTDRIAKYNQLLRIEDELFET AKYDGIKSFYNLDK
Expression Region
1-434aa
Sequence Info
Full Length
Source
E.coli
Tag Info
N-terminal 6xHis-SUMO-tagged
MW
63.1 kDa
Alternative Name(s)
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Relevance
Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.
Reference
Whole genome sequencing of meticillin-resistant Staphylococcus aureus.Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. Hiramatsu K.Lancet 357:1225-1240(2001)
Purity
Greater than 90% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.
Subcellular Location
Cytoplasm, Secreted, Cell surface
Protein Families
Enolase family
Tag Information
N-terminal 6xHis-SUMO-tagged

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 20ug
Lieferbar: In stock
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