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Recombinant Human Acetyl-CoA acetyltransferase, mitochondrial(ACAT1)

ArtNr CSB-EP001134HU-10
Hersteller Cusabio
Menge 10ug
Kategorie
Typ Proteins Recombinant
Format Liquid or Lyophilized powder
Specific against Human
Purity Greater than 85% as determined by SDS-PAGE.
ECLASS 5.1 34160400
ECLASS 6.1 34160400
ECLASS 8.0 42020190
ECLASS 9.0 42020190
ECLASS 10.0.1 32160409
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Lieferbar
Research Areas
Metabolism
Target / Protein
ACAT1
Biologically Active
Not Test
Expression System
E.coli
Species of origin
Homo sapiens (Human)
Uniprot ID
P24752
AA Sequence
VSKPTLKEVVIVSATRTPIGSFLGSLSLLPATKLG SIAIQGAIEKAGIPKEEVKEAYMGNVLQGGEGQAP TRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQ SLMCGHQDVMVAGGMESMSNVPYVMNRGSTPYGGV KLEDLIVKDGLTDVYNKIHMGSCAENTAKKLNIAR NEQDAYAINSYTRSKAAWEAGKFGNEVIPVTVTVK GQPDVVVKEDEEYKRVDFSKVPKLKTVFQKENGTV TAANASTLNDGAAALVLMTADAAKRLNVTPLARIV AFADAAVEPIDFPIAPVYAASMVLKDVGLKKEDIA MWEVNEAFSLVVLANIKMLEIDPQKVNINGGAVSL GHPIGMSGARIVGHLTHALKQGEYGLASICNGGGG ASAMLIQKL
Tag Info
N-terminal 10xHis-tagged
Expression Region
34-427aa
Protein Length
Full Length of Mature Protein
MW
45.0kDa
Alternative Name(s)
Acetoacetyl-CoA thiolase (T2) (ACAT) (MAT)
Relevance
Plays a major role in ketone body metabolism.
Reference
Human chromosome 11 DNA sequence and analysis including novel gene identification.
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. Sakaki Y.
Nature 440:497-500(2006)
Purity
Greater than 85% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Tag Information
N-terminal 10xHis-tagged

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 10ug
Lieferbar: In stock
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