Recombinant SARS-CoV-2 S1 Subunit Protein RBD, R346K/E484K/N501Y mutant

Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Arg319-Phe541. R346K/E484K/N501Y mutant: amino acid Arg (R) at 346 position was mutated to Lys (K), Glu (E) at 484 position was mutated to Lys (K), Asn (N) at 501 position was mutated to Tyr (Y).

Human embryonic kidney 293 (HEK293) cells

Lot specific (see the label on the vial), determined by BCA protein assay

C-terminal his-tag

Figure 1. Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated. Lane 1: Protein standard ladder (kDa). Lane 2: Untreated protein Lane 3: Treated protein with deglycosylation enzymes under native conditions. Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.

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