Recombinant HCov-HKU1 S1 subunit, RBD

Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.

Supplied as a 0.2 um filtered solution in PBS (pH 7.4)

Human embryonic kidney 293 (HEK293) cells

Lot specific (see the label on the vial), determined by BCA protein assay

C-terminal his-tag

Figure 1. Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size (ˆ¼25 kDa), thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.Lane 1: protein standard ladder (kDa).Lane 2: untreated protein.Lane 3: treated protein with deglycosylation enzymes under native conditions.Lane 4: treated protein with deglycosylation enzymes under denature conditions.

We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.