ArtNr |
230-30191B-50 |
Hersteller |
Raybiotech
|
Menge |
50 ug |
Kategorie |
|
Typ |
Proteins Recombinant |
Format |
Liquid |
Applikationen |
WB, IP, ELISA, Dot |
Specific against |
other |
Purity |
>95% - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Lieferbar |
|
Application Information |
Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc. |
Storage/Stability |
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles. |
Formulation |
Supplied as a 0.2 um filtered solution in PBS (pH 7.4) |
Expressed Region |
Val16-Phe318 |
Protein Name & Synonyms |
Spike protein, S Protein, S1 Subunit |
Expression System |
Human embryonic kidney 293 (HEK293) cells |
Purification |
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
Concentration (lot specific) |
Lot specific (see the label on the vial), determined by BCA protein assay |
Endotoxin Level |
< 0.5 EU per µg of the protein as determined by the LAL method |
Conjugation/Tag Notes |
C-terminal his-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
Molecular Weight (kDa) |
Recombinant protein product has a calculated molecular mass of ∼35 kDa. Due to the abundant glycosylation, it migrates as approximately 50-55 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one reduced ∼35 kDa band. See deglycosylation analysis image below. |
Shipping |
Ice packs |
Protein Type |
Recombinant Protein |
Conjugation |
Biotin |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.