Recombinant HBV A 2 Envelope Protein

Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.

Lyophilized from a 0.2 um filtered solution in PBS (pH 7.4)

HBV Large Envelope Protein (B1 subtype), L glycoprotein, L-HBsAg, Large S protein

His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)

Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.

C-terminal his-tag

Figure 1. Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size (ˆ¼25 kDa), thus indicating that the untreated recombinant protein (Lane 2, ˆ¼30 kDa) was glycosylated.Lane 1: protein standard ladder (kDa).Lane 2: untreated protein.Lane 3: treated protein with deglycosylation enzymes under native conditions.Lane 4: treated protein with deglycosylation enzymes under denature conditions.Lane 5: deglycosylation enzymes only without target proteins.

We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.