ArtNr |
230-30171-200 |
Hersteller |
Raybiotech
|
Menge |
200 ug |
Kategorie |
|
Typ |
Proteins Recombinant |
Format |
Lyophilized powder |
Applikationen |
WB, IP, ELISA, Dot |
Specific against |
Human |
Host |
Hamster - Armenian |
Konjugat/Tag |
His |
Purity |
>95% - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Lieferbar |
|
Gene Symbols |
SERPINF2 |
Application Information |
Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc. |
Storage/Stability |
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles. |
Formulation |
Lyophilized from a 0.2 um filtered solution in PBS |
Expressed Region |
Asn40-Lys491 |
Protein Name & Synonyms |
Serpin Family F Member 2, Alpha-2-Plasmin Inhibitor, Serine (Or Cysteine) Proteinase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 2, Serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 2, ALPHA-2-PI, Alpha-2-AP, Serpin F2, PLI, AAP, A2AP, API |
Expression System |
Human embryonic kidney 293 (HEK293) cells |
Purification |
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
Concentration (lot specific) |
Determined by BCA protein assay |
Reconstitution |
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
Conjugation/Tag Notes |
N-terminal his-tag |
Molecular Weight (kDa) |
Recombinant protein product has a calculated molecular mass of 50 kDa. Due to the abundant glycosylation, it migrates as approximately 60 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under denature condition, the protein presented as one single 50 kDa band. See deglycosylation analysis in SDS-PAGE image. |
SDS-PAGE Image |
Figure 1. Deglycosylation of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.Lane 1: Protein standard ladder (kDa) Lane 2: Untreated protein under reducing conditions.Lane 3: Treated protein with deglycosylation enzymes under native conditions.Lane 4: Treated protein with deglycosylation enzymes under denature conditions.Lane 5: Deglycosylation mixture only without target proteins. |
Shipping |
Ice packs |
Custom Labeling Request |
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now. |
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