Vergleich

Recombinant Human CTLA4, Biotinylated

ArtNr 230-30167B-10
Hersteller Raybiotech
Menge 10 ug
Quantity options 10 ug 200 ug 50 ug 10 ug 100 ug 200 ug 50 ug
Kategorie
Typ Proteins Recombinant
Format Lyophilized
Applikationen WB, IP, ELISA, Dot
Specific against Human (Homo sapiens)
Host Human
Konjugat/Tag Biotin, HIS
Purity >95% - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Applications
Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Manufacturer - Category
Proteins
Manufacturer - Conjugate / Tag
N-terminal histidine tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method.
Storage Conditions
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C.   For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.
Molecular Weight
Recombinant protein has a calculated molecular weight of about 14 kDa. Due to the abundant glycosylation, it migrates as approximately 18-22 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Gene Symbols
CD152CTLA4
Formulation
Lyophilized from a 0.2 um filtered solution in PBS
Expressed Region
Lys36-Asp161
Protein Name & Synonyms
Cytotoxic T-lymphocyte protein 4 (Cytotoxic T-lymphocyte-associated antigen 4) (CTLA-4) (CD antigen CD152)
Format
Lyophilized powder
Purification
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration
Determined by Pierce BCA protein assay kit (lot specific)
Reconstitution
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
Endotoxin Level
Not determined
Preservative
None

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 10 ug
Lieferbar: Out of stock
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