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Recombinant Sus scrofa FABP3

ArtNr 230-30146-10
Hersteller Raybiotech
Menge 10 ug
Kategorie
Typ Proteins Recombinant
Format Lyophilized powder
Applikationen WB, IP, ELISA, Dot
Specific against other
Host Hamster - Armenian
Konjugat/Tag His
Purity >95% - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Lieferbar
Gene Symbols
FABP3
Application Information
Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C.  For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS
Expressed Region
Val2-Ala133
Protein Name & Synonyms
Fatty acid-binding protein, heart, Fatty Acid Binding Protein 3, Mammary-Derived Growth Inhibitor, Fatty Acid Binding Protein 3, Muscle And Heart, Heart-Type Fatty Acid-Binding Protein, Muscle Fatty Acid-Binding Protein, Fatty Acid Binding Protein 11, FABP11, H-FABP, M-FABP, MDGI, Fatty Acid Binding Protein 3, Muscle And Heart (Mammary-Derived Growth Inhibitor), Fatty Acid-Binding Protein 3, O-FABP.
Expression System
Human embryonic kidney 293 (HEK293) cells
Purification
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration (lot specific)
Determined by BCA protein assay
Reconstitution
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
Conjugation/Tag Notes
N-terminal his-tag
Molecular Weight (kDa)
Recombinant protein product has a calculated molecular mass of 15 kDa. Due to the abundant glycosylation, it migrates as approximately 18 kDa extra protein band (beside 15 kDa band) in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one single 15 kDa band. See deglycosylation analysis in SDS-PAGE image.
SDS-PAGE Image
Figure 1. Deglycosylation of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.Lane 1: protein standard ladder (kDa).Lane 2: untreated protein under reducing conditions.Lane 3: treated protein with deglycosylation enzymes under native conditions.Lane 4: treated protein with deglycosylation enzymes under denature conditions.Lane 5: deglycosylation mixture only without target proteins.
Shipping
Ice packs
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 10 ug
Lieferbar: In stock
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Lieferung vsl. bis 23.08.2024 

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