Vergleich

Recombinant Human hCD83, Biotinylated

ArtNr 230-30074B-200
Hersteller Raybiotech
Menge 200 ug
Kategorie
Typ Proteins Recombinant
Format Lyophilized powder
Applikationen WB, IP, ELISA, Dot
Specific against Human
Purity >95% - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Lieferbar
Gene Symbols
CD83
Application Information
Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C.  For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.
Formulation
Lyophilized from a 0.2 ?m filtered solution in PBS
Expressed Region
Thr20-Glu144
Protein Name & Synonyms
hCD83
Expression System
Human Embryonic Kidney 293 Cells
Purification
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration (lot specific)
Determined by Pierce BCA protein assay kit
Reconstitution
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
Preservative
None
Conjugation/Tag Notes
N-terminal his-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method.
Molecular Weight (kDa)
Recombinant Human hCD83 has a calculated molecular mass of 15 kDa. Beside one minor 15-kDa protein band, one major enlarged protein band (~18 kDa) also presented in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Shipping
Ice packs
Conjugation
Biotin

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 200 ug
Lieferbar: In stock
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