ArtNr |
230-30028B-10 |
Hersteller |
Raybiotech
|
Menge |
10 ug |
Kategorie |
|
Typ |
Proteins Recombinant |
Format |
Lyophilized powder |
Applikationen |
WB, IP, ELISA, Dot |
Specific against |
Mouse |
Konjugat/Tag |
His |
Purity |
>90% - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Lieferbar |
|
Gene Symbols |
TFF2 |
Application Information |
ELISA, EIA, protein-protein interaction studies, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array |
Storage/Stability |
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles. |
Formulation |
Lyophilized from a 0.2 um filtered solution in PBS |
Expressed Region |
Glu24-Tyr129 |
Protein Name & Synonyms |
Tff2 protein, Trefoil Factor 2, TFF2 |
Expression System |
Human Embryonic Kidney 293 Cells |
Purification |
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC) |
Concentration (lot specific) |
Determined by BCA protein assay kit (Thermo Scientific) |
Reconstitution |
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS. |
Endotoxin Level |
Not determined |
Preservative |
None |
Conjugation/Tag Notes |
N-terminal 6x histidine tag and C-terminal mouse IgG Fc tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
Molecular Weight (kDa) |
Recombinant mouse TFF2 has a calculated molecular mass of 42 kDa (including a C-terminal mouse IgG Fc tag, 27 kDa). Due to the abundant glycosylation, it migrates as approximately 45-50 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
Shipping |
Ice packs |
Protein Type |
Recombinant Protein |
Conjugation |
Biotin |
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