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HRP-SARS-CoV-2 Spike RBD (R346K, E484K, N501Y), His Tag

HRP-SARS-CoV-2 Spike RBD (R346K, E484K, N501Y), His Tag

Hersteller	ACROBiosystems
Kategorie	Peptides & Proteins Recombinant Proteins
Typ	Proteins Recombinant
Specific against	SARS-CoV-2
Format	Powder
Menge	200 ug
ArtNr	SPD-C52M4-200ug
Konjugat/Tag	HRP Labeled Protein
eClass 6.1	34160400
eClass 9.0	42020190
Lieferbar
Alias	Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD
Purity	>90% as determined by SDS-PAGE.
Bioactivity Legend 1	Detection of HRP-SARS-CoV-2 Spike RBD (R346K, E484K, N501Y), His Tag titer by ELISA Assay. Immobilized Human ACE2 / ACEH Protein, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind HRP-SARS-CoV-2 Spike RBD (R346K, E484K, N501Y), His Tag (Cat. No.SPD-C52M4) with a linear range of 0.02-0.313μg/mL (QC tested).
Bioactivity Legend 2	Detection of HRP-SARS-CoV-2 Spike RBD (R346K, E484K, N501Y), His Tag titer by compitive-ELISA Assay. Serial dilutions of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM122) (Cat. No. S1N-M12A1) were added into Human ACE2 / ACEH Protein, Fc Tag (Cat. No. AC2-H5257): HRP-SARS-CoV-2 Spike RBD (R346K, E484K, N501Y), His Tag (Cat. No.SPD-C52M4) binding reactions. The half maximal inhibitory concentration (IC50) is 0.1003 μg/mL (Routinely tested).
Buffer	PBS, pH7.4
Characteristics	This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 67.7 kDa.
Description	It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Endotoxin Level	Less than 1.0 EU per μg by the LAL method.
Exp Region	Arg 319 - Lys 537
Expression System	HEK293
Molecular Weight	67.7 kDa
Molecule	Spike RBD
Shipping condition	RT
Storage	-20℃, aviod light

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Menge: 200 ug

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Lieferung vsl. bis 26.07.2024

Express-Lieferung: 3 Tage sparen

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