ArtNr |
NKM-ATGP1609-500ug |
Hersteller |
NKMAX
|
Menge |
500ug |
Applikationen |
SDS-PAGE |
Specific against |
Human |
Konjugat/Tag |
His-Tag |
Purity |
> 90% by SDS-PAGE |
Citations |
Epstein A.C.R., et al. (2001) Cell. 107:43-54<br> Lee S., et al. (2000) Cancer Cell. 8:155-167 |
NCBI |
NP_071356 |
Alias |
Egl nine homolog 3, HIFPH3, PHD3 |
Similar products |
EGLN3, Egl nine homolog 3, PHD3, HIFPH3, ATGP1609-10ug, ATGP1609-20ug, ATGP1609-50ug, ATGP1609-100ug, ATGP1609-250ug, ATGP1609-500ug, ATGP1609-1mg, ATGP1609-10, ATGP1609-20, ATGP1609-50, ATGP1609-100, ATGP1609-250, ATGP1609-500, ATGP1609-1 |
Lieferbar |
|
Domain |
1-239aa |
Description |
EGLN3, a member of the EGLN family of prolyl hydroxylases, has been shown to catalyze hydroxylation of the alpha subunit of hypoxia-inducible factor-alpha, which targets hypoxia-inducible factor-alpha for ubiquitination by a ubiquitin ligase complex containing the von Hippel-Lindau (VHL) tumor suppressor. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM2 in hypoxia, limiting glycolysis. under normoxia, hydroxylates and regulates the stability of ADRB2. Recombinant human EGLN3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. |
Concentration |
0.25mg/ml (determined by Bradford assay) |
Formulation |
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.3M NaCl, 5mM DTT, 2mM EDTA |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Manufacturers Gene Category |
Metabolism |
Molecular Weight |
29.8 kDa (263aa) confirmed by MALDI-TOF |
Bioassay Procedure |
MPLGHI MRLDLEKIAL EYIVPCLHEV GFCYLDNFLG EVVGDCVLER VKQLHCTGAL RDGQLAGPRA GVSKRHLRGD QITWIGGNEE GCEAISFLLS LIDRLVLYCG SRLGKYYVKE RSKAMVACYP GNGTGYVRHV DNPNGDGRCI TCIYYLNKNW DAKLHGGILR IFPEGKSFIA DVEPIFDRLL FFWSDRRNPH EVQPSYATRY AMTVWYFDAE ERAEAKKKFR NLTRKTESAL TED |
Expression System |
E.coli |
Manufacturers Product Type |
Recombinant Protein |
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