ArtNr |
BOS-PROTP05017-50ug |
Hersteller |
Boster
|
Menge |
50ug |
Format |
Lyophilized Powder |
Specific against |
Mouse |
Dry ice |
Yes
|
Sequence |
The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Glu-Thr-Leu. |
Alias |
Somatomedin C; IGF-I; IGFIA; IGF1 |
Lieferbar |
|
Manufacturers Product Category |
Recombinant Proteins |
Short Description |
Insulin-Like Growth Factor I mouse Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7600 Dalton. IGF-I is purified by proprietary chromatographic techniques. |
Description |
Insulin-Like Growth Factor I mouse Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7600 Dalton. IGF-I is purified by proprietary chromatographic techniques. |
Gene Name |
IGF1 |
Contents |
The protein was lyophilized with no additives. |
Bioactivity/Biological Activity |
The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is less than 1.0 ng/ml, corresponding to a specific activity of 1MU/mg. |
Purification |
Greater than 98.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE. |
Storage |
Lyophilized IGFI although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF-1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
Reconstitution |
It is recommended to reconstitute the lyophilized IGF1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Gene Full Name |
Insulin-like growth factor I |
Protein Function |
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (By similarity). Ca (2+)- dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (PubMed:21496647). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (By similarity). |
Subcellular Localization |
Secreted |
Format Information |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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