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TNF-a Tumor Necrosis Factor-Alpha Rabbit Recombinant Protein

TNF-a Tumor Necrosis Factor-Alpha Rabbit Recombinant Protein

ArtNr	BOS-PROTP04924-5ug
Hersteller	Boster
Menge	5ug
Format	Lyophilized Powder
Specific against	Rabbit
Dry ice	Yes
Sequence	The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Ala-Ser-Arg.
Alias	Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; TNF; TNFA; TNFSF2.
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Manufacturers Product Category	Recombinant Proteins
Short Description	Tumor Necrosis Factor-a Rabbit Recombinant consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. TNF-alpha was produced in E. coli is a non-glycosylated, polypeptide chain having a molecular mass of 17.4 kDa for the individual subunit. The TNF-alpha is purified by standard chromatographic techniques.
Description	Tumor Necrosis Factor-a Rabbit Recombinant consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. TNF-alpha was produced in E. coli is a non-glycosylated, polypeptide chain having a molecular mass of 17.4 kDa for the individual subunit. The TNF-alpha is purified by standard chromatographic techniques.
Gene Name	TNF
Contents	TNF-alpha Rabbit was lyophilized after extensive dialysis against 20mM PB, pH7.4, 300mM NaCl.
Bioactivity/Biological Activity	The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less than 0.03ng/ml, corresponding to a Specific Activity of 30, 000, 000 IU/mg.
Purification	Greater than 95% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.
Storage	Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Reconstitution	It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Gene Full Name	Tumor necrosis factor
Protein Function	Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Subcellular Localization	Cell membrane
Format Information	Sterile Filtered White lyophilized (freeze-dried) powder.

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Menge: 5ug

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Lieferung vsl. bis 04.10.2024

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