ArtNr |
BOS-PROTP04924-5ug |
Hersteller |
Boster
|
Menge |
5ug |
Format |
Lyophilized Powder |
Specific against |
Rabbit |
Dry ice |
Yes
|
Sequence |
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Ala-Ser-Arg. |
Alias |
Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; TNF; TNFA; TNFSF2. |
Lieferbar |
|
Manufacturers Product Category |
Recombinant Proteins |
Short Description |
Tumor Necrosis Factor-a Rabbit Recombinant consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. TNF-alpha was produced in E. coli is a non-glycosylated, polypeptide chain having a molecular mass of 17.4 kDa for the individual subunit. The TNF-alpha is purified by standard chromatographic techniques. |
Description |
Tumor Necrosis Factor-a Rabbit Recombinant consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. TNF-alpha was produced in E. coli is a non-glycosylated, polypeptide chain having a molecular mass of 17.4 kDa for the individual subunit. The TNF-alpha is purified by standard chromatographic techniques. |
Gene Name |
TNF |
Contents |
TNF-alpha Rabbit was lyophilized after extensive dialysis against 20mM PB, pH7.4, 300mM NaCl. |
Bioactivity/Biological Activity |
The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less than 0.03ng/ml, corresponding to a Specific Activity of 30, 000, 000 IU/mg. |
Purification |
Greater than 95% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE. |
Storage |
Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
Reconstitution |
It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Gene Full Name |
Tumor necrosis factor |
Protein Function |
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. |
Subcellular Localization |
Cell membrane |
Format Information |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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