ArtNr |
BOS-M01047-4 |
Hersteller |
Boster
|
Menge |
0.1mg |
Format |
Liquid |
Applikationen |
WB, FC, IP, IHC-P |
Clon |
HM47 |
Specific against |
Human, Mouse, Rat, Rabbit, Guinea Pig, Chicken, Dog, Horse, Pig, Cow, Primate |
Host |
Mouse |
Isotype |
IgG1 Kappa |
Alias |
BCR alpha, Ig-alpha, MB-1, IGA |
Lieferbar |
|
Manufacturers Product Category |
Primary Antibodies, Monoclonal Antibodies |
Short Description |
Boster Bio Anti-Hu CD79a Purified Monoclonal Antibody (Catalog# M01047-4). Tested in Flow Cytometry, IP, WB, IHC-P application(s). This antibody reacts with Dog, Chicken, Rabbit, Guinea pig, Horse, Cow, Mouse, Rat, Pig, Primate, Human. |
Description |
Boster Bio Anti-Hu CD79a Purified Monoclonal Antibody (Catalog# M01047-4). Tested in Flow Cytometry, IP, WB, IHC-P application(s). This antibody reacts with Dog, Chicken, Rabbit, Guinea pig, Horse, Cow, Mouse, Rat, Pig, Primate, Human. |
Background |
CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling. |
Gene Name |
CD79A |
Clonality |
Monoclonal |
Concentration |
1 mg/ml |
Contents |
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Application Details |
Flow cytometry: 1-4 μg/ml, intracellular staining. Western blotting: 1-2 μg/ml. |
Immunogen |
Synthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a. The mouse monoclonal antibody HM47 reacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms. |
Purification |
Purified by protein-A affinity chromatography. |
Storage |
Store at 2-8°C. Do not freeze. |
Gene Full Name |
CD79a molecule |
Molecular Weight |
25038 MW |
Protein Function |
Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src- family tyrosine kinases. Represses BCR signaling during development of immature B-cells. |
Subcellular Localization |
Cell membrane; Single-pass type I membrane protein. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts. |
Tissue Specificity |
B-cells. |
Protein Name |
B-cell antigen receptor complex-associated protein alpha chain |
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