ArtNr |
BOS-M00254-8-10ug |
Hersteller |
Boster
|
Menge |
10ug |
Format |
Lyophilized |
Applikationen |
FC, IF, IHC, ICC |
Clon |
5E12 |
Specific against |
Human |
Host |
Mouse |
Isotype |
IgG2b |
Lieferbar |
|
Manufacturers Product Category |
Primary Antibodies, Mouse Monoclonal Antibodies |
Short Description |
Boster Bio Anti-Ki67 Antibody Picoband™ (monoclonal, 5E12) catalog # M00254-8. Tested in Flow Cytometry, IF, IHC, ICC applications. This antibody reacts with Human. |
Description |
Boster Bio Anti-Ki67 Antibody Picoband™ (monoclonal, 5E12) catalog # M00254-8. Tested in Flow Cytometry, IF, IHC, ICC applications. This antibody reacts with Human. |
Background |
Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. |
Gene Name |
MKI67 |
Application Details |
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
Application Notes |
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. |
Clonality |
Monoclonal |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Contents |
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4. |
Immunogen |
E. coli-derived human Ki67 recombinant protein (Position: K2860-I3256). |
Purification |
Immunogen affinity purified. |
Storage |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
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