ArtNr |
BOS-A05089-2-10ug |
Hersteller |
Boster
|
Menge |
10ug |
Format |
Lyophilized |
Applikationen |
WB, FC, IHC, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Isotype |
IgG |
Lieferbar |
|
Manufacturers Product Category |
Primary Antibodies, Rabbit Polyclonal Antibodies |
Short Description |
Boster Bio Anti-BCAT1 Antibody Picoband™ catalog # A05089-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-BCAT1 Antibody Picoband™ catalog # A05089-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Background |
BCAT1, Branched-chain Aminotransferase1, is also know as BCT1. The BCAT1 gene is highly expressed early in embryogenesis, and during organogenesis its expression is localized to the neural tube, the somites, and the mesonephric tubules. The gene is also expressed in several MYC-based tumors. The BCAT1 gene is mapped to chromosome 12. Lack of the enzyme BCT can cause auxotroph, a kind of auxotrophic mutant in Chinese-hamster ovary cells that lacks the ability to grow if alpha-ketoisovaleric acid, alpha-ketoisocaproic acid, and alpha-keto-beta-methylvaleric acid are substituted for valine, leucine, and isoleucine in the culture medium. |
Gene Name |
BCAT1 |
Application Details |
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat Flow Cytometry, 1-3 μg/1x106 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human |
Application Notes |
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. |
Clonality |
Polyclonal |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Contents |
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4. |
Immunogen |
E.coli-derived human BCAT1 recombinant protein (Position: L88-S386). |
Purification |
Immunogen affinity purified. |
Storage |
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Reconstitution |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Molecular Weight |
43 kDa |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.