ArtNr |
BOS-A05038 |
Hersteller |
Boster
|
Menge |
0.1mg |
Format |
Liquid |
Applikationen |
WB, IF, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Isotype |
IgG |
Alias |
RILP, EPM1B, RILP, Prickle-like protein 1 |
Lieferbar |
|
Manufacturers Product Category |
Primary Antibodies |
Manufacturers Research Category |
Cancer, Cell Cycle |
Short Description |
Boster Bio Anti-Prickle-like protein 1 PRICKLE1 Antibody (Catalog # A05038). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-Prickle-like protein 1 PRICKLE1 Antibody (Catalog # A05038). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat. |
Background |
PRICKLE1, also known as RILP or EPM1B, is a Disheveled-associated protein that serves as a nuclear translocation receptor for REST/NRSF and REST4 (1, 2). It contains three N-terminal LIM domains and three C-terminal nuclear localization signals. It localizes to the cytoplasm, as well as to the nuclear membrane and expressed at higher levels in placenta. PRICKLE1 is a negative regulator of the Wnt / beta-catenin signaling pathway and is a putative tumor suppressor in human HCCs. Defects in the gene encoding PRICKLE1 are associated with autosomal recessive progressive myoclonic epilepsy. |
Gene Name |
PRICKLE1 |
Clonality |
Polyclonal |
Concentration |
1 mg/mL |
Contents |
PRICKLE1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Application Details |
PRICKLE1 antibody can be used for detection of PRICKLE1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher. |
Immunogen |
PRICKLE1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PRICKLE1. The immunogen is located within the last 50 amino acids of PRICKLE1. |
Cross-reactivity |
Multiple isoforms of PRICKLE1 are known to exist. PRICKLE1 antibody is predicted to not cross-react with other PRICKLE protein family members. |
Purification |
PRICKLE1 Antibody is affinity chromatography purified via peptide column. |
Storage |
PRICKLE1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Gene Full Name |
Prickle homolog 1 |
Molecular Weight |
94300 MW |
Protein Function |
Involved in the planar cell polarity pathway that controls convergent extension during gastrulation and neural tube closure. Convergent extension is a complex morphogenetic process during which cells elongate, move mediolaterally, and intercalate between neighboring cells, leading to convergence toward the mediolateral axis and extension along the anteroposterior axis. Necessary for nuclear localization of REST. May serve as nuclear receptor. |
Subcellular Localization |
Nucleus membrane |
Tissue Specificity |
Expressed at highest levels in placenta and at lower levels in lung, liver, kidney and pancreas. Expressed in thalamus, hippocampus, cerebral cortex, and cerebellum (in neurons rather than glia). |
Protein Name |
Prickle homolog 1 |
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