ArtNr |
BOS-A02432 |
Hersteller |
Boster
|
Menge |
0.1mg |
Format |
Liquid |
Applikationen |
WB, IF, ELISA, IHC-P |
Specific against |
Human |
Host |
Rabbit |
Isotype |
IgG |
Alias |
Bcl-2-modifying factor |
Lieferbar |
|
Manufacturers Product Category |
Primary Antibodies |
Manufacturers Research Category |
Apoptosis |
Short Description |
Boster Bio Anti-Bcl-2-modifying factor Bmf Antibody (Catalog # A02432). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human. |
Description |
Boster Bio Anti-Bcl-2-modifying factor Bmf Antibody (Catalog # A02432). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human. |
Background |
Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program. Bmf is constitutively expressed in many tissues. |
Gene Name |
BMF |
Clonality |
Polyclonal |
Concentration |
1 mg/mL |
Contents |
Bmf Antibody is supplied in PBS containing 0.02% sodium azide. |
Application Details |
Bmf antibody can be used for detection of Bmf by Western blot at 2.5 and 5 μg/mL. A band at approximately 25 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL. Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher. |
Immunogen |
Bmf antibody was raised with a synthetic peptide corresponding to 14 amino acids near the carboxy terminus of human Bmf. The immunogen is located within the last 50 amino acids of Bmf. |
Purification |
Bmf Antibody is affinity chromatography purified via peptide column. |
Storage |
Bmf antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Gene Full Name |
Bcl2 modifying factor |
Molecular Weight |
20508 MW |
Protein Function |
May play a role in apoptosis. Isoform 1 seems to be the main initiator. |
Subcellular Localization |
Isoform 2: Mitochondrion membrane ; Single-pass membrane protein. Nucleus . |
Tissue Specificity |
Isoform 1 is mainly expressed in B-lymphoid cells. Isoform 2 and isoform 3 are mainly expressed in B-CLL and normal B-cells. |
Protein Name |
Bcl2 modifying factor |
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