ArtNr |
BOS-A01252-2-10ug |
Hersteller |
Boster
|
Menge |
10 ug |
Format |
Lyophilized |
Applikationen |
FC, IF, IHC, ICC, ELISA |
Specific against |
Human (Homo sapiens) |
Host |
Rabbit |
Isotype |
IgG |
Citations |
1. Dimitrova, N., de Lange, T. MDC1 accelerates nonhomologous end-joining of dysfunctional telomeres. Genes Dev. 20: 3238-3243, 2006. 2. Goldberg, M., Stucki, M., Falck, J., D'Amours, D., Rahman, D., Pappin, D., Bartek, J., Jackson, S. P. MDC1 is required for the intra-S-phase DNA damage checkpoint. Nature 421: 952-956, 2003. |
Alias |
Mediator of DNA damage checkpoint protein 1; Nuclear factor with BRCT domains 1; MDC1; KIAA0170; NFBD1 |
Lieferbar |
|
Manufacturer - Category |
Primary Antibodies |
Manufacturer - Isotype |
Rabbit IgG |
Storage Conditions |
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. |
Observed Molecular Weight |
50 kDa |
Clonality |
Polyclonal |
Application Details |
Immunohistochemistry (Paraffin-embedded Section), 0.5-1?g/ml
Immunocytochemistry/Immunofluorescence, 2?g/ml
Flow Cytometry (Fixed), 1-3?g/1x106 cells
ELISA, 0.1-0.5?g/ml |
Manufacturer - Gene Name |
MDC1 |
Gene Full Name |
mediator of DNA damage checkpoint 1 |
Manufacturer - Specificity |
No cross reactivity with other proteins. |
Background |
Mediator of DNA damage checkpoint protein 1 is a 2080 amino acid long protein that in humans is encoded by the MDC1 gene. The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. |
Immunogen |
E. coli-derived human MDC1 recombinant protein (Position: A534-Q626). |
Contents |
Each vial contains 4 mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3. |
Purification |
Immunogen affinity purified. |
Reconstitution |
Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml. |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. |
Manufacturer - Research Category |
DNA/RNA, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling |
Protein Function |
Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. |
Subcellular Localization |
Nucleus. |
Description |
Boster Bio Anti-MDC1 Antibody Picoband® catalog # A01252-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. |
Tissue Specificity |
Highly expressed in testis. |
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