ArtNr |
BOS-A00740 |
Hersteller |
Boster
|
Menge |
0.1mg |
Format |
Liquid |
Applikationen |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse |
Host |
Rabbit |
Isotype |
IgG |
Alias |
PPBL, BENTA, BIMP3, IMD11, CARMA1, Caspase recruitment domain-containing protein 11, CARD-containing MAGUK protein 1, Carma 1 |
Lieferbar |
|
Manufacturers Product Category |
Primary Antibodies |
Manufacturers Research Category |
Apoptosis |
Short Description |
Boster Bio Anti-CARMA1 CARD11 Antibody (Catalog # A00740). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse. |
Description |
Boster Bio Anti-CARMA1 CARD11 Antibody (Catalog # A00740). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse. |
Background |
CARMA proteins belong to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. Both CARMA1 and CARMA3 associate with NEMO, the regulatory subunit of the IκK complex, thereby regulating activation of the NF-κB transcription factor. Also, gene inactivation studies showed a complete block in T and B cell immunity as well as an impaired response to LPS, indicating that CARMA1 is a critical regulator in both the adaptive and innate immune systems. |
Gene Name |
CARD11 |
Clonality |
Polyclonal |
Concentration |
1 mg/mL |
Contents |
CARMA1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Application Details |
CARMA1 antibody can be used for detection of CARMA1 by Western blot at 0.5 to 2 μg/mL. A band at approximately 125 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher. |
Immunogen |
CARMA1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARMA1. The immunogen is located within the last 50 amino acids of CARMA1. |
Purification |
CARMA1 Antibody is affinity chromatography purified via peptide column. |
Storage |
CARMA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Gene Full Name |
Caspase recruitment domain family, member 11 |
Molecular Weight |
133284 MW |
Protein Function |
Involved in the costimulatory signal essential for T- cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10. Also activates the TORC1 signaling pathway. |
Subcellular Localization |
Cytoplasm |
Tissue Specificity |
Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL- 60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLaS3, MOLT-4, A-549 and G431 cells. |
Protein Name |
Caspase recruitment domain family, member 11 |
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