ArtNr |
BOS-A00526-1 |
Hersteller |
Boster
|
Menge |
0.1mg |
Format |
Liquid |
Applikationen |
WB, IF, ICC, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Isotype |
IgG |
Alias |
IBD10, WDR30, APG16L, ATG16A, ATG16L, UNQ9393/PRO34307, Autophagy-related protein 16-1, APG16-like 1 |
Lieferbar |
|
Manufacturers Product Category |
Primary Antibodies |
Manufacturers Research Category |
Autophagy |
Short Description |
Boster Bio Anti-ATG16 ATG16L1 Antibody (Catalog # A00526-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-ATG16 ATG16L1 Antibody (Catalog # A00526-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat. |
Background |
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist. |
Gene Name |
ATG16L1 |
Clonality |
Polyclonal |
Concentration |
1 mg/mL |
Contents |
ATG16 Antibody is supplied in PBS containing 0.02% sodium azide. |
Application Details |
ATG16 antibody can be used for the detection of ATG16 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher. |
Immunogen |
ATG16 antibody was raised against a 18 amino acid synthetic peptide from near the center of human ATG16. The immunogen is located within amino acids 190 - 240 of ATG16. |
Purification |
ATG16 Antibody is affinity chromatography purified via peptide column. |
Storage |
ATG16 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Gene Full Name |
ATG16 autophagy related 16-like 1 (S. cerevisiae) |
Molecular Weight |
68265 MW |
Protein Function |
Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane (PubMed:24553140, PubMed:23376921, PubMed:24954904, PubMed:27273576, PubMed:23392225). Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (PubMed:25645662). Negatively regulates NOD1- and NOD2- driven inflammatory cytokine response (PubMed:24238340). Instead, promotes with NOD2 an autophagy-dependent antibacterial pathway (PubMed:20637199). Plays a role in regulating morphology and function of Paneth cell (PubMed:18849966). |
Subcellular Localization |
Cytoplasm |
Tissue Specificity |
Ubiquitous. |
Protein Name |
ATG16 autophagy related 16-like 1 (S. cerevisiae) |
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