ArtNr |
BOS-A00312-1 |
Hersteller |
Boster
|
Menge |
100 ug/vial |
Format |
Lyophilized |
Applikationen |
WB, FC, IHC, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Lieferbar |
|
Manufacturers Product Category |
Primary Antibodies, Rabbit Polyclonal Antibodies |
Short Description |
Boster Bio Anti-STIM1 Antibody Picoband™ catalog # A00312-1. Tested in ELISA, IHC, WB, Flow Cytometry (Intracellular) applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-STIM1 Antibody Picoband™ catalog # A00312-1. Tested in ELISA, IHC, WB, Flow Cytometry (Intracellular) applications. This antibody reacts with Human, Mouse, Rat. |
Background |
Stromal interaction molecule 1 is a protein that in humans is encoded by the STIM1 gene. STIM1 has a single transmembranedomain, and is localized to the endoplasmic reticulum, and to a lesser extent to the plasma membrane. This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. |
Gene Name |
STIM1 |
Application Details |
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry, 2-5 μg/ml, Human, Mouse, Rat Flow Cytometry (Intracellular), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, Human |
Application Notes |
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. |
Clonality |
Polyclonal |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Contents |
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4. |
Immunogen |
E.coli-derived human STIM1 recombinant protein (Position: E42-L599). Human STIM1 shares 98.2% and 98% amino acid (aa) sequence identity with mouse and rat STIM1, respectively. |
Purification |
Immunogen affinity purified. |
Storage |
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Reconstitution |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Molecular Weight |
85 kDa |
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