ArtNr |
BOS-A00146-1 |
Hersteller |
Boster
|
Menge |
0.1mg |
Format |
Liquid |
Applikationen |
WB, IF, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
Isotype |
IgG |
Alias |
ALS9, RAA1, HEL168, RNASE4, RNASE5, Angiogenin, Ribonuclease 5, RNase 5 |
Lieferbar |
|
Manufacturers Product Category |
Primary Antibodies |
Manufacturers Research Category |
Cancer, Cell Cycle |
Short Description |
Boster Bio Anti-Angiogenin ANG Antibody (Catalog # A00146-1). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-Angiogenin ANG Antibody (Catalog # A00146-1). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat. |
Background |
Angiogenin (ANG or ANG I) is important for the process of neovascularization and formation of new blood vessels. ANG is similar to pancreatic ribonuclease A and functions as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. It interacts with endothelial cell-surface actin and may cause changes in the cell cytoskeleton. ANG is thought to be involved in the development of solid tumors and its antagonists are capable of inhibiting tumor growth. Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9). Angiogenin is a genetic link between ALS and PD. |
Gene Name |
ANG |
Clonality |
Polyclonal |
Concentration |
1 mg/mL |
Contents |
ANG Antibody is supplied in PBS containing 0.02% sodium azide. |
Predicted Reactivity |
Horse |
Application Details |
ANG antibody can be used for detection of ANG by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher. |
Immunogen |
ANG antibody was raised against a 15 amino acid synthetic peptide near the center of human ANG. The immunogen is located within amino acids 70 - 120 of ANG. |
Cross-reactivity |
Two alternatively spliced transcript variants have been observed. |
Purification |
ANG Antibody is affinity chromatography purified via peptide column. |
Storage |
ANG antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Gene Full Name |
Angiogenin, ribonuclease, RNase A family, 5 |
Molecular Weight |
16550 MW |
Protein Function |
Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. |
Subcellular Localization |
Cytoplasmic vesicle, secretory vesicle lumen |
Tissue Specificity |
Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. |
Protein Name |
Angiogenin, ribonuclease, RNase A family, 5 |
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