In vivo Collagen Hybridizing Peptide, sulfo-Cy7.5 Conjugate

The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains, both in vitro and in vivo.[1, 2, 3] By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature.

The in vivo CHP incorporates our newest sequence which allows them to be directly injected into animals without having to pre-activate them with a heating step. [4] Therefore, the in vivo CHPs will always be in their active form and will not self-hybridize once injected. [4] The sCy7.5-CHP is a powerful in vivo tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging.[3] CHP can measure and localize mechanical injury to collagenous tissue at the molecular level.[5]

The sCy7.5-CHPs are labeled with sulfonated-cyanine 7.5 dye for near-infrared fluorescence (NIRF) detection. This wavelength offers better tissue penetration while minimizing tissue autofluorescence.

in vivo imaging, small animal imaging

New sequence designed for in vivo use- does not need any pre-activation step unlike CHPs for histology
Near-infrared imaging probe minimizes the chance of autofluorescence in the gut or certain tissues
Can be used for systemic or in situ injection
Signal lasts at target sites for days, allowing for imaging at multiple time points with a single dose
High affinity and unparalleled specificity to collagen turnover with essentially no nonspecific binding
Applicable to all types of collagen from all species, relying on collagen's secondary structure instead of any defined sequence for binding
Stable as powder form, stored at -20 °C

90% by HPLC

797 nm

-20 °C as powder (4 °C for a few days once reconstituted)