Recombinant mouse GAPDH protein

Mouse

Specific activity is > 40 units/mg, and is defined as theamount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1, 3-Bisphosphoglycerate per minute at pH 8.5 at 37C.

Gapdh, also known as glyceraldehyde 3-phosphate dehydrogenase, is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, Gapdh has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport. Recombinant mouse Gapdh, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

E.coli

Enzymes & Proteases

38.2kDa (356aa) confirmed by MALDI-TOF